Leaf Chlorophylls and Carotenoids Status and Their Correlation with Storage Root Weight of Some Local and Exotic Sweetpotato Genotypes

Main Article Content

Md. Abu Shahadat Hossain
A. F. M. Saiful Islam
Mohammad Noor Hossain Miah
Mohammad Mehedi Hasan Khan

Abstract

The investigation was carried out to characterize the chlorophyll components and carotenoids of the leaves of some local and exotic genotypes of sweetpotato viz. Local-1, Local-2, Local-5, Local-8, Exotic-1, Exotic-2, Exotic-4 and BARI SP-4 and their effect on production of total dry matter and storage roots dry weight during November 2016 to March 2017 at farmer’s field of Dashpara village of Sylhet Sadar Upazila, Sylhet, Bangladesh. The experiment was laid out in Randomized Complete Block Design with three replications. Fresh leaves of 5-6th position from the top of vine were collected from the research field into polybag with proper tagging and brought to the laboratory in the morning of 30, 60, 90 and 120 days after planting (DAP). Collected leaves were washed, wiped out of excess water, cut into small pieces, mixed thoroughly, and 250 mg of leaf materials were taken in a mortar. Leaf materials were grinded finely by a pestle with 25 ml of cold 80% acetone for two minutes. Sample tubes were centrifuged for 10 minutes. The homogenate was filtered and made up to 25 ml with cold 80% acetone. The centrifuged samples were incubated in dark for half an hour. The optical density (OD) for each solution was measured at 663, 645 and 440.5 nm against 80% acetone as blank in one cm cell of spectrophotometer. Triplicate estimation was done for each sample. Chemical analyses were performed at Regional Laboratory of Soil Resource Development Institute, Sylhet. Statistical analyses was done using MSTATC software following analysis of variance technique and Duncan’s Multiple Range Test. Results showed that chlorophyll-a gradually increased up to 60 DAP in all genotypes, thereafter continued only in Exotic-4, Exotic-3 and Local-1 up to 90 DAP. The highest amount of chlorophyll-a (10.27±0.45 mg 100 gfw-1) was in Local-1 at 90 DAP. The highest amount of chlorophyll-b was in Exotic-3 (19.13±0.53 mg 100 gfw-1) followed by Local-1 (16.85±0.50 mg 100 gfw-1) at 30 DAP. Carotenoids content in leaves of all genotypes increased gradually up to 90 DAP and thereafter decreased except Exotic-4. The highest carotenoids was in Exotic-3 (10.78 mg 100 gfw-1) followed by Local-1 (10.13 mg 100 gfw-1) at 90 DAP. At 120 DAP, the highest storage roots weight was in Local-8 (232.40±5.97 g plant-1), followed by Local-1 (187.50±5.23 g plant-1). Chlorophylls and carotenoids had no significant effect on total dry matter and storage roots dry weights at 30 DAP. At 120 DAP, all chlorophyll components and carotenoids had positive correlation with total dry matter (TDM) and storage roots dry weights. Genotypes Local-1, Local-8 had the higher chlorophylls while Exotic-3, Local-1 and Local-8 had the higher carotenoids. Genotypes Local-1 and Local-8 showed the highest storage roots dry weight.

Keywords:
Chlorophylls, carotenoids, exotic genotype, local genotype, storage root, correlation.

Article Details

How to Cite
Hossain, M. A. S., Islam, A. F. M., Miah, M. N., & Khan, M. M. (2019). Leaf Chlorophylls and Carotenoids Status and Their Correlation with Storage Root Weight of Some Local and Exotic Sweetpotato Genotypes. Asian Journal of Research in Botany, 2(4), 1-11. Retrieved from http://journalajrib.com/index.php/AJRIB/article/view/30074
Section
Original Research Article

References

Ministry of Agriculture, Government of Bangladesh. Crop situation in Bangladesh; 2017.
Available:www.moa.gov.bd,
http//F:/sweetpotato/information/crop_situation_2014-16.pdf

Food and Agriculture Organization. FAO Statistical Year Book of the United Nations, Regional Office for Asia and the Pacific Bangkok. 2014;176.
Available:www.fao.org

Kozai T, Kubota C and Kitaya Y. Sweetpotato technology for solving the global issues on food, energy, natural resources and environment in the 21st century. Environ. Control in Biol. 2006;34: 105–114.

Woolfe JA. Sweetpotato: An untapped food resource, Cambridge University Press, Cambridge, UK. 1992;643.

Laurie SM, Faber M, van Jaarsveld PJ, Laurie RN, du Plooy CP, Modisane PC. Beta-carotene yield and productivity of orange-fleshed sweetpotato [Ipomoea batatas (L.) Lam.] as influenced by irrigation and fertilizer application treatments. Sci. Hortic. 2012;142:180-184.

Islam S. Nutritional and medicinal qualities of sweetpotato tops and leaves. Coopera- tive Extension Program, University of Arkansas, Pine Bluff, Chicago, USA; 2014.

Arnon DI. Copper enzyme in isolated chloroplast polyphenol oxidase in Beta vulgaris (L.). Plant Physiol. 1949;24:1-5.

Abramavicius D, Valkunas L. Role of coherent vibrations in energy transfer and conversion in photosynthetic pigment-protein complexes. Photosynth. Res. 2016; 127:33–47.

Batjuka A, Skute N, Petjukevics A. The influence of anthocyanin on pigment composition and functional activity of photosynthetic apparatus of Triticum aestivum L. under high temperature. Photosynthetica. 2016;55:1–14.

Zakar T, Laczko-Dobos H, Toth T N, Gombos Z. Carotenoids assist in cyanobacterial photosystem II assembly and function. Front. Plant Science. 2016; 7:295.

Campos MD, Nogales A, Cardoso HG, Campos C, Grzebelus D, Velada I, Arnholdt-Schmitt B. Carrot plastid terminal oxidase gene (dcptox) responds early to chilling and harbors intronic pre-mirnas related to plant disease defense. Plant Gene. 2016;7:21–25.

Santabarbara S, Casazza AP, Ali K, Economou CK, Wannathong T, Zito F, Redding KE, Rappaport F, Purton S. The requirement for carotenoids in the assembly and function of the photosynthetic complexes in chlamydomonas reinhardtii. Plant Physiol. 2013;161:535–546.

Nagy L, Kiss V, Brumfeld V, Osvay K, Borzsonyi A, Magyar M, Szabo T, Dorogi M, Malkin. Thermal effects and structural changes of photosynthetic reaction centers characterized by wide frequency band hydrophone: Effects of carotenoids and terbutryn. Photochem. Photobiol. 2015; 91:1368–1375.

Kopsell DA, Kopsell DE, Curran-Celentano J. Carotenoid and chlorophyll pigments in sweet basil grown in the field and greenhouse. HortScience. 2005;40(5): 1230–1233.

Rumbaoa RG, Cornago DF and Geronimo IM. Phenolic content and antioxidant capacity of Philippine sweetpotato [Ipomoea batatas (L.)Lam.] varieties. Food Chem. 2009;113:1133–1138.

Hue SM, Boyce AN, Chandran S. Influence of growth stage and variety on the pigment levels in Ipomoea batatas (L.) Lam. (sweetpotato) leaves. African Journal of Agricultural Research. 2011;6(10):2379-2385.

Rajput A, Rajput SS, Jha G. Physiological parameters leaf area index, crop growth rate, relative growth rate and net assimilation rate of different varieties of rice grown under different planting geometries and depths in SRI. Int. J. Pure App. Biosci. 2017;5(1):362-367.
DOI:http://dx.doi.org/10.18782/2320-7051.2472

Soil Resource Development Institute (SRDI). Land and soil statistical appraisal book of Bangladesh, 1st edition, Ministry of Agriculture, Government of Bangladesh. Dhaka. 2010;11-103.

Mackinney G. Criteria for purity of chlorophyll preparations. Journal of Biological Chemistry. 1940;132:91-109.

Maclachlan S, Zalik S. Plastid structure, chlorophyll concentration, and free amino acid composition of a chlorophyll mutant of barley. Canadian Journal of Botany. 1963; 41:1053-1062.

Duxbury AC, Yentsch CS. Plant and pigment monography. Journal of Air Pollution and Control Assessment. 1956; 16:145-150.

Holm G. Chlorophyll mutations in Barley. Acta Agric. (Scand.). 1954;4:457-461.

Katayama Y, Shida S. Studies on the change of chlorophyll a and b contents due to projected materials and some environmental conditions. Cytologia. 1970; 35:171-180.

Yooyongwech S, Samphumphuang T, Theerawitaya C, Chaum S. Physio-morphological responses of sweetpotato [Ipomoea batatas (L.) Lam.] genotypes to water-deficit stress. J. Plant Omics. 2014; 7(5):361-368.

Rashid MMHA. Comparative growth and yield studies of some exotic and local cultivars of sweetpotato. M.Sc. (Agriculture) Thesis, Dept. of Crop Botany, Bangladesh Agricultural University, Mymensingh; 2002.

Motsa NM, Modi AT, Mabhaudhi T. Influence of agro-ecological production areas on antioxidant activity, reducing sugar content, and selected phytonutrients of orange-fleshed sweetpotato cultivars. Food Sci. Technology). 2015;35(1):32-37.

Hossain MAS, Islam AFMS. Comparative study on dry matter partitioning in five exotic genotypes and five local varieties of sweetpotato. J. Sher-e-Bangla Agric. University. 2010;4(1):24-30.

Nandi S, Sen H. Evaluation of sweetpotato (Ipomoea batatas L. Lam.) cultivars under late planted situation. Root Crops J. 1998; 24(1):73-77.

Nair GM, Nair VM. Influence of irrigations and fertilizers on the growth attributes of sweetpotato. Journal of Root Crops. 1995; 21:17-23.

Mannan MA, Bhuiyan MKR, Quasem A, Rashid MM, Siddique MA. Study on the growth and partitioning of dry matter in sweet potato. J. Root Crops. 1992;18:1–5.

Haque MM. Productivity of Maize / sweetpotato intercropping in relation to planting system, population density and application of nitrogen and potassium fertilizers, PhD Dissertation, Dept. of Agronomy, Institute of Post-Graduate Studies in Agriculture, Salna, Gazipur-1703, Bangladesh. 1995;164.

Watson DJ. The dependence of net assimilation rate on leaf area index. Ann. Bot. 1958;22:37-54.

Oswald A, Alkamper J, Midmore DJ. The effect of different shade levels on growth and tuber yield of sweetpotato, 1. Plant development. Journal Agron. Crop Sci. 1994;175:99-107.