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Poulk plant (Stachys schtschegleevii Sosn. ex Grossh.) is one of the medicinal plants with a long history in traditional medicine and is used to remedy many diseases. Polk plant possesses some properties, including the internal anti-infectious, antibacterial, Anti-asthma, anti-sinusitis, anti-inflammatory and it is used to remedy the respiratory inflammatory diseases and has been identified asa natural penicillin. This study aims to investigate the phytochemical and antibacterial effects of ethanolic extract, 2-Propanol and n-hexane of the Poulk plant against Escherichia coli and Staphylococcus aureus, Bacillus subtilis and Salmonella enterica. In this study, extracting was done by Soxhlet extractor with ethanolic, 2-Propanol and n-hexane solvents and after evaporation of the solvent and methylation by the rotary evaporator, the obtained substance was injected into the GC-MASS and the substance was detected, as well as the Inhibition zone, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) studies were carried out. The ranges of MIC and MBC of ethanolic extract, 2-Propanol and n-hexane for MIC were 0.78-12.5%, 0.78 -6.25% and 12.5-50%, respectively. MBC for ethanolic extract, 2-Propanol and n-Hexane were 0.78-12.5%, 0.78 -6.25% and 12.5-100%, respectively.
Pehlivan M, Sevindik M. Antioxidant and antimicrobial activities of Salvia multicaulis. Turkish Journal of Agriculture-Food Science and Technology. 2018;6(5):628-631.
Mohammed FS, Akgül H, Sevindik M, Khaled MHT. Phenolic content and biological activities of Rhus coriaria var. zebaria. Fresen Environ Bull. 2018;27(8):5694-5702.
Pehlivan M, Mohammed FS, Sevindik M, Akgul H. Antioxidant and oxidant potential of Rosa canina. Eurasian Journal of Forest Science. 2018;6(4):22-25.
Chitsaz M, Mohammadi H, Nasseri M, Kamali Nejad M. Investigation of antibacterial effects of poulk plant (Stachys schegleevii) in-vitro conditions. Two-monthly Scientific Journal of Shahed University. 2006;14:8-1.
Bayat A, Shahanipour K. The antibacterial effect of methanolic and hydro bromic extract of poulk plant leaves on generative bacteria of urinary tract infection. Journal of Qom Medical Sciences University. 2013;9:39-43.
Ahmadzadeh CH. Investigation of antibacterial effect of artemisia plant extract on Staphylococcus aureus and Escherichia coli pathogenic bacteria. Research project of Azad University, Ahar Branch; 2013.
Mabberley DJ. Mabberley's plant-book: A portable dictionary of plants, their classifications and uses. 3rd ed. New York: Cambridge University Press; 1997.
Mazaffarian V.A dictionary of Iranian plant names. Tehran Farhang-Moaser Pub; 1996.
Kumarasamy¬¬ Y, Fergusson M, Nahar L, Sarker SD. Biological activity of moschamindole from Centaurea moschata. Q pharm Biol. 2002;40:307-10.
Maleki N, Garjani A, Nazemieh H, Nilfouroushan¬ N, Eftekhar Sadat AT, Allameh Z. Potent anti-inflamatory activities of hydroalocholic extract for aerial parts of Stachys inflata on rats. J Ethnopharmacol. 2001;75:213-8.
Rezazadeh S, Kebryaeezadeh A, Pirali-Hamedani M, Shafiee A, Isfahani SG. Anti-inflammatory andanalgesic activity of methanolic extracts of aerial parts of Stachys schtschegleevii Sosn and Stachys balansae Boiss and Kotschy ex Boiss in rats. DARU. J. 2005;13:165-9.
Mariotti JP, Costa¬ J, Bianchini A, Ber-nardini AF, Casanov, J. Composition and variability of the essential oil of species from Greece. Phytochemistry. 2003;64: 743-52.
Metscher LA, Drake S, Goliapudi SR, Okwute SK. A modern look at folkloric use of anti – infective agents. Journal of natural products. 1981;50:1025-1040.
Oussalah M, Caillet S, Saucier L, Lacroix M. Inhibitory effects of selected plant essential oils on the growth of four pathogenic bactevica E. coli: O157:H7, Listeria monocytogenes 2812 1/2a, Salmonella typhimurium SL 1344 and Staphylococcus aureus¬. Food Contvel. 2007;18:414-20.
Sevindik M. Investigation of antioxidant/ oxidant status and antimicrobial activities of Lentinus tigrinus. Advances in Pharmacological Sciences; 2018.
Sevindik M. Investigation of oxidant and antioxidant status of edible mushroom Clavariadelphus truncatus. Mantar Dergisi. 2018;9(2):165-168.
Shopsin B, Kreiswith BN. Molecular epid emiology of methicillin–resistant Staphylococcus aureus. Emerg. Infect Dis. 2001;7:323-326.
Tiemersma EW, Bronzwaer SL, Lyytikäinen O, Degener JE, Schrijnemakers P, Bruinsma N, Monen J, Witte W, Grundman H.¬ European anti-microbial resistance surveillance system participants. 1999-2002. Methicillin-resistant Staphylococcus aureus in Europe. 2004;10:1627– 634.
Senser B, Koseoglu D, Ozcelik U, Kocagoz T, Gunalp, A. Epidemiology of chronic Pseudomonas aeruginosa infections in cystic fibrosis. Int. J. Med. Microbiol. 2001;291:387-393.
Nazemiyeh Maleki N, Mehmani F¬, Kumarasamy Y, shoeb M, Garjani A, Sarkers D. Assessment of anti- inflammatory properties of ethyl acetate extract of Stachys schtschegleevii sosn. DARU. 2007;15:174-182.
Sonboli A, Salehi P, Nejad Ebrahimi S. Essential oil composition and antibacterial activity of the leaves¬ of Stachys¬¬ schtschegleevii FROM IRAN. Chemistry of Natural Compounds. 2005; 41:171-174.
Maleki-Dizaji N, Nazemiyeh¬ H, Maddah¬ N, Mehmani¬ F, Garjani A. Scerrning of extracts and fractions from aerial parts of Stachys schegleevii sosn for anti-inflammatory activitys. Pak. J. Pharm Sci. 2008;21:338-343.
Azami S, Fahimi B, Bagheri ¬M, Mohsenzadeh SH. The comparison of antibacterial effect of Schrophularia striata Boiss¬ and Stachys¬ cht¬schegleevii sosn. Extracts on pathogens isolated from urinary tract in fections. J. Herbal Drugs. 2016;7:15-20.