Asian Journal of Research in Botany <p style="text-align: justify;"><strong>Asian Journal of Research in Botany</strong>&nbsp;aims to publish high-quality papers (<a href="/index.php/AJRIB/general-guideline-for-authors">Click here for Types of paper</a>) in all aspects of Botany. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer-reviewed, open access INTERNATIONAL journal.&nbsp;</p> Asian Journal of Research in Botany en-US Asian Journal of Research in Botany Contribution to the Study of Leaves: Azadirachta indica A. Juss (Meliaceae): Evaluation of the Antioxidant Activity <p>In Africa, the use of plants for therapeutic purposes is an ancient practice. In recent years, much scientific work has been spent to the chemistry and toxicology of medicinal plants; there is a particular focus on natural antioxidants in relation to their various therapeutic properties. Therefore, the purpose of our study is to determine the antioxidant activity of aqueous and hydroethanol extracts from the leaves of <em>Azadirachta indica</em> A. Juss a plant known for its many pharmacological properties.</p> <p>The leaves of <em>Azadirachta indica </em>A. Juss were oven dried at 60 degrees for 24 hours and reduced to a fine powder. And, the powder is first extracted with distilled water to obtain the aqueous extract, then with a mixture of distilled water and ethanol 50/50 (v / v) to obtain the hydroethanol extract. Antioxidant activity was done through the DPPH test, the FRAP method and the fixation of the radical nitro-oxide (NO).</p> <p>The results revealed that for the DPPH test, the hydro-ethanol extract is more active (IC<sub>50</sub>=9.9±0.14 mcg/ml) compared to the activity of the water extract (IC<sub>50</sub>= 11±0.28 mcg/ml). For the FRAP method, we note absorbance of 0.56 and 1.05 respectively for water and hydro-ethanol extract at a concentration (166.7 µg/ml). On the other hand, for the inhibition of radical nitro-oxide (NO), activity is low for the two extracts of <em>Azadirachta indica</em> A. Juss respectively of 36.94±2.1% for the aqueous extract and 26.03±2.52% for the hydroethanol extract.</p> <p>This work highlights the antioxidant properties of Azadirachta indica A. Juss leaf extracts. Which give credit to certain data ethnopharmacological uses of <em>Azadirachta indica </em>A. Juss, but, study benefits must be carried out to support this use especially on toxicology.</p> Kady Diatta William Diatta Alioune Dior Fall Serigne Ibra Mbacké Dieng Amadou Ibrahima Mbaye Apéli Adjoa Jennifer Akpoto-Kougblenou Emmanuel Bassène ##submission.copyrightStatement## 2019-08-26 2019-08-26 1 8 Influence of Date of Sowing and Different Levels of Phosphorus on Growth and Yield of Garden Pea (Pisum sativum L.) <p>The experiment was carried out at the Horticulture Farm of Sher-e-Bangla Agricultural University, Dhaka during October 2017 to February 2018 to find out the growth, yield and economic benefit of garden pea as influenced by date of sowing and different levels of phosphorus. The research comprised of two factors: Factor A: Sowing time (three levels) as S<sub>1</sub>=15 November, S<sub>2</sub>=25 November, S<sub>3</sub>=5 December and Factor B: Phosphorus fertilizer (four levels) as P<sub>0</sub>= Control (No Phosphorus), P<sub>1</sub>=50 kg P<sub>2</sub>O<sub>5</sub>/ha, P<sub>2</sub>=75 kg P<sub>2</sub>O<sub>5</sub>/ha, P<sub>3</sub>=100 kg P<sub>2</sub>O<sub>5</sub>/ha.&nbsp; The experiment was set up in randomized complete block design (RCBD) with three replications. Sowing time and phosphorus influenced significantly on most of the parameters. Sowing time, S<sub>2 </sub>(25 November) performed best in number of pods per plant (12.10), number of seeds per pod (4.62) and green pod yield (8.48 ton) per hectare and minimum in S<sub>3</sub> (5 December) treatment. Application of phosphorus, P<sub>2 </sub>(75 kg P<sub>2</sub>O<sub>5</sub>) performed best in number of pods per plant (12.70), number of seeds per pod (4.90) and green pod yield (9.23 ton) per hectare and minimum in P<sub>0 </sub>(control) treatment. Among the treatment combination S<sub>2</sub>P<sub>2 </sub>treatment gave the highest green pod yield (10.50 t/ha) and the lowest (4.48 t/ha) was obtained from S<sub>3</sub>P<sub>0</sub> treatment. Combination of 25 November sowing with 75 kg P<sub>2</sub>O<sub>5</sub>was the best for growth, pod formation and seed formation of garden pea. From the economic point of view, the highest Benefit Cost Ratio (BCR) was (2.35) noted from S<sub>2</sub>P<sub>2 </sub>and the lowest (1.02) from S<sub>3</sub>P<sub>0</sub>. It was evident that the S<sub>2</sub>P<sub>2</sub> gave the best performance for the growth, yield and economic benefit of garden pea. So, it was concluded that, the combination of 25 November sowing with 75 kg P<sub>2</sub>O<sub>5</sub> can be used for commercial garden pea production.</p> Md. Nahidul Islam Khaleda Khatun Tahmina Mostarin Md. Ehsanul Haq Md. Rafiqul Islam Bithi Rani Biswas Jinia Afsun Md. Ashraf Ali ##submission.copyrightStatement## 2019-08-27 2019-08-27 1 13 Leaf Chlorophylls and Carotenoids Status and Their Correlation with Storage Root Weight of Some Local and Exotic Sweetpotato Genotypes <p>The investigation was carried out to characterize the chlorophyll components and carotenoids of the leaves of some local and exotic genotypes of sweetpotato viz. Local-1, Local-2, Local-5, Local-8, Exotic-1, Exotic-2, Exotic-4 and BARI SP-4 and their effect on production of total dry matter and storage roots dry weight during November 2016 to March 2017 at farmer’s field of Dashpara village of Sylhet Sadar Upazila, Sylhet, Bangladesh. The experiment was laid out in Randomized Complete Block Design with three replications. Fresh leaves of 5-6th position from the top of vine were collected from the research field into polybag with proper tagging and brought to the laboratory in the morning of 30, 60, 90 and 120 days after planting (DAP). Collected leaves were washed, wiped out of excess water, cut into small pieces, mixed thoroughly, and 250 mg of leaf materials were taken in a mortar. Leaf materials were grinded finely by a pestle with 25 ml of cold 80% acetone for two minutes. Sample tubes were centrifuged for 10 minutes. The homogenate was filtered and made up to 25 ml with cold 80% acetone. The centrifuged samples were incubated in dark for half an hour. The optical density (OD) for each solution was measured at 663, 645 and 440.5 nm against 80% acetone as blank in one cm cell of spectrophotometer. Triplicate estimation was done for each sample. Chemical analyses were performed at Regional Laboratory of Soil Resource Development Institute, Sylhet. Statistical analyses was done using MSTATC software following analysis of variance technique and Duncan’s Multiple Range Test. Results showed that chlorophyll-a gradually increased up to 60 DAP in all genotypes, thereafter continued only in Exotic-4, Exotic-3 and Local-1 up to 90 DAP. The highest amount of chlorophyll-a (10.27±0.45 mg 100 gfw<sup>-1</sup>) was in Local-1 at 90 DAP. The highest amount of chlorophyll-b was in Exotic-3 (19.13±0.53 mg 100 gfw<sup>-1</sup>) followed by Local-1 (16.85±0.50 mg 100 gfw<sup>-1</sup>) at 30 DAP. Carotenoids content in leaves of all genotypes increased gradually up to 90 DAP and thereafter decreased except Exotic-4. The highest carotenoids was in Exotic-3 (10.78 mg 100 gfw<sup>-1</sup>) followed by Local-1 (10.13 mg 100 gfw<sup>-1</sup>) at 90 DAP. At 120 DAP, the highest storage roots weight was in Local-8 (232.40±5.97 g plant<sup>-1</sup>), followed by Local-1 (187.50±5.23 g plant<sup>-1</sup>). Chlorophylls and carotenoids had no significant effect on total dry matter and storage roots dry weights at 30 DAP. At 120 DAP, all chlorophyll components and carotenoids had positive correlation with total dry matter (TDM) and storage roots dry weights. Genotypes Local-1, Local-8 had the higher chlorophylls while Exotic-3, Local-1 and Local-8 had the higher carotenoids. Genotypes Local-1 and Local-8 showed the highest storage roots dry weight.</p> Md. Abu Shahadat Hossain A. F. M. Saiful Islam Mohammad Noor Hossain Miah Mohammad Mehedi Hasan Khan ##submission.copyrightStatement## 2019-09-03 2019-09-03 1 11