Asian Journal of Research in Botany

Apomixis in Coscinium fenestratum (Gaertn.) Colebr.

C. R. Chitra *

Division of Conservation Biology, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram – 695562, India.

K. S. Jayakumar

Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram – 695562, India.

C. Anilkumar

Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram – 695562, India.

*Author to whom correspondence should be addressed.

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Abstract

Coscinium fenestratum is getting endangered due to the extensive exploitation for medicinal use. Irrespective to the profuse seed set, occurrence of seedlings in the wild is meager that prompted a seed study. As a dioecious liana, female plants bear fruits even in the absence of male plants in the vicinity and also the bagged unopened flower buds develop in to viable seeds. Absence of pollinators and air born pollen grains, development of single seed from tricarpillary gynoecium, all attributed for an enigmatic development. In consequence of curiosity, studies on pollen viability, pollen germination, pollen ovule ratio and embryological studies etc were conducted. Embryo formation was observed with 80% of the longitudinal microtome sections taken with 1^st to 3^rd week aged fruit primordia irrespective to the lack of pollen on the stigma and pollen tube entry into the stylar tube. Comparative molecular studies on mother plant and respective F1 seedlings revealed genetic identity that confirmed apomixis.

Keywords: Apomixis, Coscinium fenestratum, DNA isolation, ISSR analysis, pollen–ovule ratio, pollination

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References

Warrier PK, Nambiar VPK, Ramankutty C. Indian medicinal plants—A compendium of 500 species. Orient Longman Limited, 160. Anna Salai, Madras. 1994;2:191-193.

Birdsall, T.C.N.D.; Kelly, G.S.N.D. Berberine: Therapeutic potential of an alkaloid found in several medicinal plants. Altern. Med Review. 1997;207:94–103.

Ravikumar K, Ved DK. 100 Red listed medicinal plants of conservation concern in Southern India. Foundation for revitalization of local health traditions, Bangalore. India. 2000:99-103.

Udayan PS, Thushar KV, George S. Notes on rare, endemic, red listed plants as addition to the flora of Cannore district (Kannur), Kerala State. Indian Journal of Forestry. 2004;27:341-346. Available: http: // www. mtnforum.org

Jayaweera DMA. Medicinal plants used in Sri Lanka. National Science Council, 47/5, Maitland Place, Colombo. 1982;4:70-71

Mathew PJ, Thomas MT. Medicinal Plant Resource of Kerala, Towards harvesting its potential, Part 1-Introduction, Published by Jawaharlal Nehru Tropical Botanic Garden and Research Institute. ISBN 81-900397-7-6,16; 2007.

Kumar S, Singh J, Shah NC, Ranjan V. Indian medicinal and aromatic plants racing genetic erosion. Edam Books International, New Delhi; 1997.

Ved D, Saha D, Ravikumar K, Hariasan K. Coscinium fenestratum, The IUCN Red List of Threatened Species; 2015.

Fournier LAO, Charpentier C. El tamaño de la muestra y la frecuencia de las observaciones en el estudio de las características fenológicas de los árboles tropicales. Turrialba. 1975;25(1):45-48.

Opler PA, Frankie GW, Baker HG. Comparative phenological studies of tree let and shrubs species in tropical wet and dry forests in the lowland of Costa Rica. Journal of Ecology. 1980;68:167 - 188.

Dafni A, Kevan PG, Husband BC. Practical pollination biology. Environquest Ltd. Ontario; 2005.

Cruden RW. Pollen-ovule ratios: a conservative indicator of breeding systems in flowering plants. Evolution. 1977;31:32-46

Shivanna KR, Rangaswamy NS. Pollen biology. A laboratory manual. New Delhi, India 1992.Narosa Publishing House.

Brewbaker J, Kwack B. The essential role of calcium ion in pollen germination and pollen tube growth. American Journal of Botany. 1963;50(9):859– 865.

Heslop-Harrison J, Heslop-Harrison Y. Evaluation of pollen viability by enzymatically induced fluorescence: Intracellular hydrolysis of fluorescein diacetate. Stain Technology. 1970;45(3):115-120.

Dafni A, Firmage D. Pollen viability and longevity: practical, ecological and evolutionary implications, In: Dafni, A, Pacini, E, and Hesse, M. editors. Pollen and pollination. Berlin: Springer. 2000:113–132.

Murray HG, Thompson WF. Rapid isolation of high molecular weight DNA. Nucleic Acids Research. 1980;8:4321-4325.

Narasimhan S, Padmesh P, Nair GM. Assessment of genetic diversity in Coscinium fenestratum. Biologia Plantarum. 2006;50:111-113.

Joshi AC, Raman Rao VV. Fruit and seed development in Tinospora cordifolia Miers without fertilization and embryo formation inside. Current Science. 1934;3:62-63.

Seshagiriah KN. Seeds and seedlings of Tinospora cordifolia Miers. Current Science. 1934;3:264.

Bahadur Singh. The seeds of Tinospora cordifolia Miers. Current Science. 1934;3:265.

Ernst, Gustav Adolf. A new case of parthenogenesis in the vegetable kingdom. Nature, 1886;34:549-552.

Sumod M, Sujanapal P, Robi AJ, Dantas KJ, Arun KR, Satthiyakumar K. Population status and distribution of Coscinium fenestratum (Gaertn.) Colebr. (Menisperemaceae) in Northern Kerala. 28th Kerala Science Congress, 2016, Extended abstracts. 2016:2499-2511.

Tiwary NK. Fruit and seed development in Tinospora cordifolia Miers Exembryonate Seeds. Current Science. 1934;3:262-264.

Asker, S.E.; Jerling, L., Apomixis in plants. CRC Press, London, 1992.

Koltunow AM. Apomixis embryo sacs and embryos formed without meiosis or fertilization in ovules. Plant Cell. 1993;5:1425-1437.

Naumova, T.N., Apomixis in Angiosperms. Nucellar and integumentary Embryony, CRC Press, Boca Raton, USA, 1993.

Forman LL. A review of the tribe Coscinieae Hook.f. &Thoms. (Menispermaceae). Kew Bulletin. 1978;32: 323-338.