Effect of Terminalia catappa Seed Extract on Liver Enzymes and Lipid Profile in Male Wistar Rat
Published: 2022-12-31
Page: 334-341
Issue: 2022 - Volume 5 [Issue 2]
Hart, Victor Opuada
Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port Harcourt, Port Harcourt, Nigeria.
Chinko, Bruno Chukwuemeka *
Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port Harcourt, Port Harcourt, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The traditional use of plant-derived natural substances has continued to gain traction in the last few decades and has gradually become the mainstay of human pharmacotherapy. The present study evaluated the effect of Terminalia catappa seed extract on the lipid profile and serum liver enzymes of male Wistar rats. Fresh, ripe fruits of T. catappa were sourced locally from the University of Port Harcourt and the dried seeds were extracted using methanol and used for the study. Fifteen (15) male Wistar rats (160 – 180g) were grouped into three (3) of five (5) animals per group and used for the study. Group I served as the control and received distilled water whiles groups II and II received 500 and 1000mg/kg of ethanolic seed extract of T. catappa (TCE) by oral gavage for twenty-eight (28) days. Animals were anaesthetized, blood was collected by cardiac puncture and analyzed for serum liver enzymes: aspartate transaminase (AST), alanine Transaminase (ALT), and alkaline phosphatase (ALP) and lipid profile: total cholesterol (TC), triglycerides (TG), high-density lipoproteins (HDL) and low-density lipoproteins (LDL) using standard laboratory methods. Results obtained from the study indicate that serum ALT and LDL were significantly reduced among rats that received TEC compared to the control (p<0.05). The study concludes that TEC exhibited possible hepatoprotective properties and modulation of lipid profile and hence may be effective against liver injury and protection against cardiovascular and neurogenerative diseases.
Keywords: Terminalia catappa, liver enzyme, lipid profile, hepatoprotection, lipid peroxidation
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Thomson LAJ, Evans B. Terminalia catappa (tropical almond). Species Profiles for Pacific Island Agroforestry. 2006;2(2): 1-20.
Anand A, Divya N, Kotti P. An updated review of Terminalia catappa. Pharmacognosy reviews. 2015;9(18):93.
Omodamiro OD, Omekara I. Evaluation of in-vitro antioxidant and In-vivo diuretic activities of ethanol leaves extract of Terminalia catappa leaves. Journal of International Research in Medical and Pharmaceutical Sciences. 2017;12(13): 94-104.
Mbah BO, Eme PE, Eze CN. Nutrient potential of Almond seed (Terminalia catappa) sourced from three states of Eastern Nigeria. African Journal of Agricultural Research. 2013;8(7):629-33.
Oboh B, Ogunkanmi B, Olasan L. Phenotypic diversity in Terminalia catappa from South Western Nigeria. Pakistan Journal of Biological Sciences: PJBS. 2008;11(1):135-8.
Gilman EF, Watson DG. Terminalia catappa tropical-almond. Gainesville: Institute of Food and Agricultural Sciences, University of Florida; 1994.
Ezeokonkwo CA, Dodson WL. The potential of Terminalia catappa (tropical almond) seed as a source of dietary protein. Journal of Food Quality. 2004; 27(3):207-19.
Terças AG, Monteiro AdS, Moffa EB, Santos JRd, Sousa EMd, Pinto AR, et al. Phytochemical characterization of Terminalia catappa linn. Extracts and their antifungal activities against candida spp. Frontiers in Microbiology. 2017;8:595.
Ihuma JO, Noel DO, Adogo LY. Antimicrobial activities of Aerminalia catappa linn. leaves extract on some selected salmonella species. Journal of Biology and Nature. 2021;13(1):35-41.
Zainudin R, Nanding A, Mohamad H, Sinsuat DRR, Abo S, Usman S, et al. Phytochemical and antimicrobial analyses of Terminalia Catappa Linn. ethanol leaf extract and its effect on treating pimple. Psychology and Education: A Multidisciplinary Journal. 2022;4(6):571-80.
Gao J, Tang X, Dou H, Fan Y, Zhao X, Xu Q. Hepatoprotective activity of Terminalia catappa L. leaves and its two triterpenoids. Journal of Pharmacy and Pharmacology. 2004;56(11):1449-55.
Lawal AR, Olayinka BU, Abdulkareem KA, Abdulra’uf LB, Murthadah RA, Kayode OV. Proximate, minerals and phytochemical evaluation of pericarp and seed of Terminalia catappa fruit. The Bioscientist Journal. 2022;10(2):191-203.
Salawu RA, Onyegbula AF, Lawal IO, Akande SA, Oladipo AK. Comparative study of the nutritional, phytochemical and mineral compositions of the nuts of tropical almond (Terminalia catappa) and sweet almond (Prunus amygdalus). Ruhuna Journal of Science. 2018;9(1):70-7.
Weerawatanakorn M, Janporn S, Ho CT, Chavasit V. Terminalia catappa Linn seeds as a new food source. Songklanakarin Journal of Science & Technology. 2015; 37(5).
Owolabi MS, Lawal OA, Ogunwande IA, Hauser RM, Setzer WN. Chemical composition of the leaf essential oil of Terminalia catappa L. growing in Southwestern Nigeria. American Journal of Essential Oils and Natural Products. 2013;1(1):51-4.
Ravi L. A review on medicinal potential of Terminalia catappa. International Journal of Green Pharmacy (IJGP). 2020; 14(03):229-33.
Ladele B, Kpoviessi S, Ahissou H, Gbenou J, Kpadonou-Kpoviessi B, Mignolet E, et al. Chemical composition and nutritional properties of Terminalia catappa L. oil and kernels from Benin. Comptes Rendus Chimie. 2016;19(7):876-83.
Ratnasooriya W, Dharmasiri M. Effects of Terminalia catappa seeds on sexual behaviour and fertility of male rats. Asian Journal of Andrology. 2000;2(3):213-9.
Katiki LM, Gomes ACP, Barbieri AME, Pacheco PA, Rodrigues L, Veríssimo CJ, et al. Terminalia catappa: chemical composition, In vitro and In vivo effects on Haemonchus contortus. Veterinary Parasitology. 2017;246:118-23.
Adu OB, Omojufehinsi M, Esanboro M, Abe D, Shofolahan A, Uzodinma E, et al. Effect of processing on the quality, composition and antioxidant properties of Terminalia catappa (indian almond) seed oil. African Journal of Food, Agriculture, Nutrition and Development. 2013;13(3).
Halilu EM, Ugwah-Oguejiofor CJ, Oduncuoğlu G, Matthias SG. Physicochemical, toxicity and antioxidant activity of Terminalia catappa kernel oil in mice. Pharmacognosy Research. 2023; 15(1):199-27.
Luka CD, Istifanus G, Olatunde A, Eli Z. Effect of aqueous seed extract of Terminalia catappa linn on some biochemical parameters in alloxan-induced diabetic rats. Journal of Applied Life Sciences International. 2017;13(3):1-8.
Venkatalakshmi P, Brindha P, Induja K. In-vitro anti oxidant and antitumor studies on Terminalia catappa bark. Aging. 2001;18:685-716.
Neelavathi P, Venkatalakshmi P, Brindha P. Antibacterial activities of aqueous and ethanolic extracts of Terminalia catappa leaves and bark against some pathogenic bacteria. Int J Pharm Pharm Sci. 2013;5(1):114-20.
Chyau CC, Tsai S-Y, Ko PT, Mau JL. Antioxidant properties of solvent extracts from Terminalia catappa leaves. Food chemistry. 2002;78(4):483-8.
Akharaiyi F, Ilori R, Adesida J. Antibacterial effect of Terminalia catappa on some selected pathogenic bacteria. Int J Pharm Biomed Res. 2011;2(2):64-7.
Nagappa AN, Thakurdesai PA, Rao NV, Singh J. Antidiabetic activity of Terminalia catappa Linn fruits. Journal of ethnopharmacology. 2003;88(1):45-50.
Evans WC. Trease and Evans' Pharmacognosy. 16th ed. New York: Elsevier health sciences; 2009.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry. 1972; 18(6):499-502.
Albus U. Guide for the care and use of laboratory animals (8th edn). SAGE Publications Sage UK: London, England; 2012.
Benjamin B. Overview of laboratory animal lifestyle, care, and management: a case study of albino rats. Journal of Applied Sciences and Environmental Management. 2019;23(8):1431-5.
Atanasov AG, Waltenberger B, Pferschy-Wenzig E-M, Linder T, Wawrosch C, Uhrin P, et al. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology advances. 2015;33(8):1582-614.
Ugoeze KC, Oluigbo KE, Chinko BC. Phytomedicinal and nutraceutical benefits of the GC-FID quantified phytocomponents of the aqueous extract of Azadirachta indica leaves. Journal of Pharmacy and Pharmacology Research. 2020;4(4):149-63.
García-Niño WR, Zazueta C. Ellagic acid: Pharmacological activities and molecular mechanisms involved in liver protection. Pharmacological Research. 2015;97:84-103.
Gowda S, Desai PB, Hull VV, Avinash AK, Vernekar SN, Kulkarni SS. A review on laboratory liver function tests. The Pan african medical journal. 2009;3.
Macpherson I, Abeysekera KWM, Harris R, Mansour D, McPherson S, Rowe I, et al. Identification of liver disease: why and how. Frontline Gastroenterology. 2022; 3(5).
Chinko BC, Pughikumo DT. Haematological and hepatorenal alterations induced by potash (Akanwu) on male wistar rats. International Blood Research & Reviews. 2023;14(1):38-46.
Roy A, Bhoumik D, Sahu RK, Dwivedi J. Medicinal plants used in liver protection- A review. Pharmaceutical and Biosciences Journal. 2014;2(1):23-33.
Pan X, Ma X, Jiang Y, Wen J, Yang L, Chen D, et al. A comprehensive review of natural products against liver fibrosis: flavonoids, quinones, lignans, phenols, and acids. Evidence-based complementary and alternative medicine. 2020;2020.
Wang Y, Zhang N, Zhou J, Sun P, Zhao L, Zhou F. Protective effects of several common amino acids, vitamins, organic acids, flavonoids and phenolic acids against hepatocyte damage caused by alcohol. Foods. 2022;11(19):3014.
Tang X-h, Gao J, Wang Y-p, Xu L-z, Zhao X-n, Xu Q. Hepatoprotective effects of chloroform extract from leaf of Terminalia catappa in relation to the inhibition of liver IL-6 expression. Zhongguo Zhong yao za zhi= Zhongguo Zhongyao Zazhi= China Journal of Chinese Materia Medica. 2003;28(12):1170-4.
Tang XH, Gao J, Dou H, Wang YP, Xu LZ, Zhu ZR, et al. Protective effect of the extract of Terminalia catappa leaves on acute liver injury induced by D-GalN in mice. Zhongguo Zhong yao za zhi= Zhongguo Zhongyao Zazhi= China Journal of Chinese Materia Medica. 2004; 29(11):1069-73.
Gaschler MM, Stockwell BR. Lipid peroxidation in cell death. Biochemical and biophysical research communications. 2017;482(3):419-25.
Repetto M, Semprine J, Boveris A. Lipid peroxidation: Chemical mechanism, biological implications and analytical determination. Lipid peroxidation. 2012;1:3-30.
Singh R, Devi S, Gollen R. Role of free radical in atherosclerosis, diabetes and dyslipidaemia: larger‐than‐life. Diabetes/metabolism research and reviews. 2015;31(2):113-26.
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. The international journal of biochemistry & cell biology. 2007;39(1):44-84.
Banjarnahor SD, Artanti N. Antioxidant properties of flavonoids. Medical Journal of Indonesia. 2014;23(4):239-44.
Karak P. Biological activities of flavonoids: An overview. Int. J. Pharm. Sci. Res. 2019;10(4):1567-74.
Terao J. Dietary flavonoids as antioxidants. Food factors for health promotion. 2009;61:87-94.
Zeka K, Ruparelia K, Arroo RR, Budriesi R, Micucci M. Flavonoids And Their Metabolites: Prevention In Cardiovascular Diseases And Diabetes. Diseases. 2017; 5(3):1-18.
You CL, Su PQ, Zhou XX. Study on effect and mechanism of scutellaria baicalensis stem-leaf total flavonoid in regulating lipid metabolism. China Journal of Chinese Materia Medica. 2008;33(9):1064-6.
Barbalho SM, Damasceno DC, Spada APM, Silva VSd, Martuchi KA, Oshiiwa M, et al. Metabolic profile of offspring from diabetic Wistar rats treated with Mentha piperita (peppermint). Evidence-Based Complementary and Alternative Medicine. 2011;2011.
Alor PC, Chinko BC. Flat tummy water attenuates lipid profile and serum glucose of high-fat diet-induced obese female wistar rats. Journal of Complementary and Alternative Medical Research. 2022;20(4):19-28.
Ikete PW, Chinko BC. Inhibition of serum lipase as a mechanism of action for anti-obesity potentials of Dioscorea bulbifera extracts on wistar rats. Asian Journal of Health Sciences. 2022;8(1):ID29-ID.
Mukundan U, Sharma A. Anti-obesity and anti-hyperlipidemic activity Of Terminalia catappa linn. in high-fat-diet induced obese rats. Asian Journal of Pharmaceutical Research and Development. 2013; 1(6):114-20.
Ambidevi S. Anti-obesity and anti-hyperlipidemic activity of Terminalia catappa linn. high-fat-diet indiuced obese rats. Asian Journal of Pharmaceutical Research and Development, Mumbai. 2014;1(6).