The Phylogenetic Relationships between Species of Vicia L. Based on Morphological Characteristics and Proteins Present in Seeds
Published: 2023-10-03
Page: 225-232
Issue: 2023 - Volume 6 [Issue 2]
Alaa Ayman Hasan *
Department of Botany, Faculty of Science, Tishreen University, Latakia, Syria.
Dina Ali Haddad
Department of Botany, Faculty of Science, Tishreen University, Latakia, Syria.
Latifeh Mohammed Ali
Department of Botany, Faculty of Science, Tishreen University, Latakia, Syria.
*Author to whom correspondence should be addressed.
Abstract
Fabaceae is the second most economically important family of flowering plants all over the world. Its species are used widely as food and fodder crops because of their high protein content.
Four species of Vicia L. (vetches) were selected in this research to determine the phylogenetic relationships among Spp. by comparing the molecular characteristics, specifically the protein content, with the morphological ones. The species were Vicia sativa L. (Common Vetch), Vicia galeata Boiss. (Helmeted Vetch), Vicia palaestina Boiss. (Palestine vetch), and Vicia hybrida L. (Hairy yellow vetch). They were collected from the gardens of Tishreen University in Latakia – Syria. Two dendrograms were constructed depending on 35 differences in morphological characteristics and 30 bands of seed protein content resulting from Sodium Dodecyl Sulfate-Polyacrylamide GEL Electrophoresis (SDS-PAGE) method.
The results showed that the dendrograms of both morphological and molecular characteristics were similar. Both of them consisted of three clusters. The first cluster included V.palaestina, that was the most distant genetically from the rest of the species, the second one contained V.sativa, whereas the third cluster showed a strong phylogeny between V.galeata and V.hybrida.
This type of studies is important to determine phylogenetic relationships among species and classifying them due to the lack of reported researches locally.
For our knowledge it was a first time that the total protein content of V.palaestina was studied.
Keywords: Fabaceae, SDS-PAGE, protein content, dendrogram, morphology, globulin
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References
Raveendar S, Lee GA, Jeon YA, Lee YJ, Lee JR, Cho GT, et al. Cross-amplification of Vicia sativa subsp. sativa microsatellites across 22 other Vicia species. Molecules. 2015;20(1):1543-1550.
Food and Agriculture Organization of the United Nations (FAO). Production/Yield quantities of Vetches in Syrian Arab Republic1994 – 2021. 2023. Accessed on 27 May 2023 Available at: https://www.fao.org/faostat/en/#data/QCL/visualize.
Gepts P, Beavis WD, Brummer EC, Shoemaker RC, Stalker HT, Weeden NF, et al. Legumes as a model plant family. Genomics for food and feed report of the cross-legume advances through genomics conference. 2005;137:1228-1235.
Post GE. Flora of Syria, Palestine and Sinai. Vol I. 2nd ed. Ed. Dimsmore JE. American University of Beirut; 1932.
Mouterde P. Nouvelle flore du Liban et de la Syrie. 2nd ed. Beirut, Lebanon: Dar el-Mashreg; 1970. French.
Warsame AO, O’Sullivan DM, Tosi P. Seed storage proteins of faba bean (Vicia faba L): Current status and prospects for genetic improvement. Journal of Agricultural and Food Chemistry. 2018; 66(48):12617-12626.
Muzquiz M, Varela A, Burbano C, Cuadrado C, Guillamón E, Pedrosa MM. Bioactive compounds in legumes: Pronutritive and antinutritive actions. Implications for nutrition and health. Phytochemistry reviews. 2012;11:227-244.
Das Gupta S, Suh N. Tocopherols in cancer: An update. Molecular nutrition & food research. 2016;60(6):1354-1363.
Salehi B, Abu‐Reidah IM, Sharopov F, Karazhan N, Sharifi‐Rad J, Akram M, Pezzani R. Vicia plants—A comprehensive review on chemical composition and phytopharmacology. Phytotherapy Research. 2021;35(2):790-809.
Abozeid A, Liu Y, Liu J, Tang Z. Comparative foliar structure of vicia l. species from China. Journal of Biosciences and Medicines. 2017a;5(03):170-175.
Abozeid A, Liu Y, Liu J, Tang Z. Cluster analysis of leaf macro-and micro-morphological characteristics of Vicia L.(Fabaceae) and their taxonomic implication. Phyton. 2017b;86:306-317.
Haider AS, El-Shanshoury AR. Variability of storage proteins and esterase isozymes in Vicia sativa subspecies. Biologia plantarum. 2000;43:205-209.
Azat A, Baboujian G. A study of the protein content of seeds of some types of alfalfa Medicago spread in Quneitra. Damascus University Journal of Basic Sciences. 2012;28(2):461-474.
Khalik KNA, Al Gohary IH. Taxonomic relationships in some Vicia species from Egypt, based on seed morphology and SDS-PAGE of seed proteins. Acta Scientiarum. Biological Sciences. 2013; 35(4):603-611.
Jukanti AK, Dagla HR, Kalwani P, Goswami D, Upendra JM, Kalia RK, et al. Grain protein estimation and SDS-PAGE profiling of six important arid legumes. Legume Research-An International Journal. 2017;40(3):485-490.
Ladizinsky G, Hymowitz T. Seed protein electrophoresis in taxonomic and evolutionary studies. Theoretical and Applied Genetics. 1979;54:145-151.
Mirali N, El-Khouri S, Rizq F. Genetic diversity and relationships in some Vicia species as determined by SDS-PAGE of seed proteins. Biologia Plantarum. 2007; 51(4):660-666.
Celebi A, Acik L, Aytac Z. Biosystematics studies among Ebenus L. Species based on morphological, RAPD-PCR and seed Protein analyses in Turkey. Pak. J. Bot. 2009;41(5):2477-2486.
Hameed A, Shah TM, Atta BM, Iqbal N, Haq MA, Ali H. Comparative seed storage protein profiling of Kabuli chickpea genotypes. Pak. J. Bot. 2009;41(2):703-710.
Emre I, Turgut-Balik D, Genc H, Sahin A. Total seed storage protein patterns of some Lathyrus species growing in Turkey using SDS-PAGE. Pak. J. Bot. 2010; 42(5):3157-3163.
Jafri SMH. Flora of Libya: Fabaceae. Tripoly, Libya: al-Faateh university., Department of botan; 1980.
Davis PH. Flora of Turkey and the East Aegean Island. 8th ed. Turkey: Edinburgh University, press; 1984.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-685.
Han S, Sebastin R, Lee KJ, Wang X, Shin MJ, Kim SH, et al. Interspecific variation of seed morphological and micro‐morphological traits in the genus Vicia (Fabaceae). Microscopy Research and Technique. 2021;84(2):337-357.
Elamine Y, Torres‐Salas V, Messai A, Girón‐Calle J, Alaiz M, Vioque J. Purification, characterization, and antiproliferative activity of a single‐chain lectin from Vicia palaestina (Fabaceae) seeds. Chemistry & Biodiversity. 2021; 18(2):e2000827.
Emre I. Determination of genetic diversity in the Vicia L.(section vicia) by using SDS-PAGE. Pak. J. Bot. 2011;43(3):1429-1432.
Caracuta V, Weinstein-Evron M, Kaufman D, Yeshurun R, Silvent J, Boaretto E. 14,000-year-old seeds indicate the Levantine origin of the lost progenitor of faba bean. Scientific reports. 2016;6(1): 37399.
Siddique KHM, Loss SP, Enneking D. Narbon bean (Vicia narbonensis L.): a promising grain legume for low rainfall areas of south-western Australia. Australian Journal of Experimental Agriculture. 1996;36(1):53-62.
Nagel S, Matic R, Kirby G. Vetch in Australian farming systems. Eyre Peninsula Farm. Syst. 2011;71-72.
Megías C, Cortés-Giraldo I, Giron-Calle J, Alaiz M, Vioque J. Free amino acids, including canavanine, in the seeds from 32 Vicia species belonging to subgenus Vicilla. Biocatalysis and Agricultural Biotechnology. 2016;8:126-129.
Kaplan A, Ertekin AS, Gündüzer E. Molecular Phylogenetic Analyses of Vicia L.(Fabaceae) Taxa Growing in the Southeastern Anatolia Region Based on Chloroplast TrnL Sequences. International Journal of Nature and Life Sciences. 2021a;5(1):11-22.
Kaplan, A, Ertekin, AS, Gündüzler, E. Molecular phylogenetic analysis of Vicia L. (Fabaceae) taxa growing in the Southeastern Anatolia region of Turkey: based on internal transcribed spacer (ITS). Turkish Journal of Agriculture-Food Science and Technology. 2021b;9(10): 1831-1839.