Antifungal Activities of Non-volatile Compounds of Trichoderma harzianum and Trichoderma longibrachiatum against Rot Fungi Associated with Ananas comosus Linn. (Pineapple)
Published: 2024-04-22
Page: 94-101
Issue: 2024 - Volume 7 [Issue 1]
Adewuyi-Samuel Oluwatoke Bolatito *
Biology Department, The Polytechnic, Ibadan, Ibadan, Oyo State, Nigeria.
Fatoki Oladotun Ayodele
Biology Department, The Polytechnic, Ibadan, Ibadan, Oyo State, Nigeria.
Alagbe Gbolahan Oluwasegun
Biology Department, The Polytechnic, Ibadan, Ibadan, Oyo State, Nigeria.
Olubisi Oluwasegun John
Institute of Agricultural Research and Training, Obafemi Awolowo University, Moor Plantation, Ibadan, Oyo State Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aim: This study investigated the inhibitory effect of non-volatile compounds of Trichoderma harzianum and Trichoderma longibrachiatum against fungal pathogens associated with spoilt pineapple.
Study Design: This experiment was laid out in a randomized complete block design.
Place and Duration of Study: Department of Biology, The Polytechnic, Ibadan, Nigeria, between June 2023 and September 2023
Methodology: Pineapples exhibiting symptoms of rot were purchased from Oje market within Ibadan, Nigeria. Isolation of rotten fungi was done by direct inoculating samples showing symptoms of rot on sterilized Potato Dextrose agar (PDA). Fungal isolates were identified using molecular techniques. The role of the isolates obtained in a formation of symptoms was determined by the pathogenicity test. Evaluation of non-volatile antifungal compounds of T. harzianum and T. longibrachiatum against the fungal isolates were carried out in-vitro at 15%, 20% and 25% concentrations. All the data obtained were subjected to statistical analysis.
Results: The mycoflora isolated was identified as Penicillium funiculosum, Aspergillus niger and Meyerozyma carribica. The isolates reproduced the same rot symptoms, while A. niger and P. funiculosum had a high level of virulence with increasing incubation times. The non-volatile compounds of both biocontrol agents exert fungistatic effect on A. niger, M. carribica and P. funiculosum at 15%, 20% and 25% concentrations.
Conclusion: Therefore, non-volatile compounds of both biological agents inhibited growth of pathogenic fungi of pineapple in vitro. Thus, it could serve as a better alternative to synthetic chemicals.
Keywords: Fungistatic effect, mycoflora, non-volatile compounds, pathogenicity test
How to Cite
Downloads
References
Liu D, Coloe S, Baird R, Pedersen J. Rapid mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology. 2017;381: 471.
Chillet M, Hoareau A, Hoarau M, Minier J. Potential Use of Essentials Oils to Control Fruitlet Core Rot (FCR) in Pineapple (Queen Victoria Variety) in Reunion Island. American Journal of Plant Sciences 2020;11:1671-1681.
Food and Agricultural Organization of United State. Yearbook; 2019. Website: www. fao. org.
Quijandria G, Berrocal J, Lawrence P. “La industria de la pina en Costa Rica: Analisis de sostenibilidad. Centro Centroamericano dr desarrollo Sostenible”; 1997. Available:http://www.incae.edu/EN/clads/publicaciones/pdf/Cen707.pdf
All Africa. Horticulture Can Provide Three Million Jobs in Few Year; 2011. Available:http://allafrica.com/stories/201111180784.html?page=2
Moss MO. Mycotoxin review journal on Aspergillus penicillium. Mycologist. 2002;16:116-119.
Zhang LU, McCarthy MJ. Black heart characterization and detection in pomegranate using NMR relaxometry and MR imaging. Postharvest Biol. Technol. 2017;67:96–101.
Zakawa NN, Timon D, Yusuf CS, Tizhe TD, Bala UJ, Isa A, Waja S, Aphonsus G. Isoltion and Control of Fungal Rot Pathogen of Tomato Fruit Using Aqueous Leaf Extracts of Azadirachta indica in Mubi, Adamawa State. Research Journal of Plant Pathology. 2019;2:1-5
Onuoroh S C, Udemezue OF, Uche JC, Okoli IC. Fungi Association with the spoilage of Pineapple Fruits in Eke Awka Market Anambra State. The Bioscientist. 2013;1:22-27
Bhardwaj NR, Kumar J. Characterization of volatile secondary metabolite s from Trichoderma asperellum. Journal of Applied and Natural Science. 2017:9:954-959
Odebode AC, Joseph CC, Jonker SA, Wachira. SW. Antifungal activities of constituents from Uvaria schefferi and Abotrys brachpetalas. Journal of Agricultural Science. 2006; 51: 79-86.
Michaelsen A, Pinzari F, Ripka K, Lubitz W, Pinar G. Application of molecular techniques for identification of fungal communities colonizing paper material. International Biodeterioration and Biodegradation. 2006;58:33-141.
Okoro CC, Amund OO, Eliora R. 2009. Plasmids of phenanthrene and dibenzothiophene-degrading bacteria isolated from produced water samples in oil production. Int. J. Biol. Chem Sci. 2009;3:186-191.
Chukwuka KS, Okonko IO, Adekunle AA. Microbial ecology of organisms causing pawpaw (Carica papaya L.) Fruit decay in Oyo State, Nigeria. American-Eurasian J.Toxicol. Sci. 2010;2:43-50.
Dennis C, Webster J. Antagonistic properties of species-groups of Trichoderma, I. Production of non-volatile antibiotics. Transactions of the British Mycological Society. 1971a;57:41-48
Oniah T, Tawose FO. Fungi associated with black rot disease of pineapple (Ananas comosus L.) fruits and the effects of the disease on nutritional value o the fruits. ISABB Journal of Food and Agricultural Science 2018; 8(3):18-24.
Limtong S, Kaewwichian R, Yongmanitchai W, Kawasaki H. Diversity of culturable yeasts in phylloplane of sugarcane in Thailand and their capability to produce indole-3-acetic- acid. World J. Microbiol Biotechnol. 2014;30:1785-1796
Michailides TJ, Morgan DP, Quist M, Reyes HC. Infection pomegranate by Alternaria sp. causing blackheart. Available:http://ucanr.edu/sites/Pomegranat es/files/122813.pdf. 2012
Bastien B, Marc C, Anna D, Maeva G, Laetitia R, Mathieu L, Noel D, Lindy JR, Altus V, Sabine Schorr- G. Diversity and Toxigenicity of Fungi that Cause Pineapple Fruitlet Core Rot. Toxins 2020;5(12):339-342
Hanan IM, and Mohamed OI. The efficacy of non-volatile compound of Trichoderma species and Bacillus isolates in the control of chickpea wilt pathogens. Agriculture, Forestry and Fisheries. 2014;3:346-351
Khaledi N, Taheri P. Biocontrol mechanisms of Trichoderma harzianum against soybean charcoal rot caused by Macrophomina phaseolina. Journal of plant protection research. 2016; 56:21-31.
Waseem R, Muhammad F, Sohail Y, Faheem UR, Muhammad Y. Volatile and nonvolatile antifungal compounds produced by Trichoderma harzianum SQR-T037 suppressed the growth of Fusarium oxysporum f. sp. niveum. Science letters. 2013;1(1):21-24.
Ítalo TSRM, Vanderly AS, Giovana DD, Spartaco AF, Edson JC, Marcos JSV. Yeasts with Fermentative Potential Associated with Fruits of Camu-Camu (Myrciaria dubia, Kunth) from North of Brazilian Amazon. The Scientific World Journal. 2021; 5:1-6.
Raza W, Faheem M, Yousuf S, Rayar UF, Yameen M. Volatile and non-volatile antifungal compounds produced by Trichoderma harzianum SQR-T037 suppressed the growth of Fusarium oxysporum f. sp. niveum. Science Letters. 2013;1:21-24.