Comprehensive Pharmacological Assessment and Phytochemical Analysis of Tetrastigma angustifolia (Roxb.) Deb.: Unveiling Analgesic, Anxiolytic and Hypoglycemic Potential in Traditional Medicinal Contexts

Jannatul Mysha Binte Quader

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh.

Ummah Tasnim Nisat

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh and Department of Pharmacy, University of Science and Technology, Chittagong-4202, Bangladesh.

Anik Kumar Nath

Department of Pharmacy, BGC Trust University Bangladesh, Kanchannagar-4381, Bangladesh.

Cinmoy Barua

Department of Pharmacy, BGC Trust University Bangladesh, Kanchannagar-4381, Bangladesh.

Kaniz Fatema Bristy

Department of Pharmacy, BGC Trust University Bangladesh, Kanchannagar-4381, Bangladesh.

Munjerin Sultana

Department of Pharmacy, BGC Trust University Bangladesh, Kanchannagar-4381, Bangladesh.

Muradur Rahman

Department of Pharmacy, BGC Trust University Bangladesh, Kanchannagar-4381, Bangladesh.

Mohi Uddin *

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh.

*Author to whom correspondence should be addressed.


Abstract

Aims: Despite its promising applications in traditional medicine, the bioactivity of Tetrastigma angustifolia and its constituent phytochemicals remain underexplored. This study sought to elucidate the potential therapeutic benefits of Tetrastigma angustifolia in animal models, specifically focusing on its analgesic, anxiolytic and hypoglycemic properties. Additionally, a comprehensive analysis of the plant’s chemical composition was conducted.

Methodology: Methanolic extracts of Tetrastigma angustifolia leaves were subjected to phytochemical screening. The anxiolytic effects were assessed through the Elevated Plus Maze and Hole Board tests. Analgesic activity was evaluated using the Acetic Acid-Induced Writhing method, while hypoglycemic effects were determined through the Glucose Tolerance Test.

Results: Phytochemical analysis identified the presence of alkaloids (0.5%), tannins (1.2%), and flavonoids (0.8%). The extract demonstrated significant anxiolytic activity, with 60% increase in time spent in the open arms of the Elevated Plus Maze compared to control groups (p < 0.01). Analgesic properties were evident with a 50% reduction in writhing response (p < 0.05). Moreover, the extract exhibited dose-dependent hypoglycemic effects, resulting in a 30% reduction in blood glucose levels (p < 0.001) relative to controls.

Conclusion: Tetrastigma angustifolia exhibits considerable therapeutic potential as evidenced by its significant analgesic, anxiolytic and hypoglycemic effects. These findings suggest its potential utility in drug development. However, to substantiate the observed effects, further research is warranted, including in silico analyses and more sophisticated experimental validations.

Keywords: Tetrastigma angustifolia, bioactivity, phytochemical analysis, analgesic activity, anxiolytic activity, hypoglycemic activity


How to Cite

Quader, Jannatul Mysha Binte, Ummah Tasnim Nisat, Anik Kumar Nath, Cinmoy Barua, Kaniz Fatema Bristy, Munjerin Sultana, Muradur Rahman, and Mohi Uddin. 2024. “Comprehensive Pharmacological Assessment and Phytochemical Analysis of Tetrastigma Angustifolia (Roxb.) Deb.: Unveiling Analgesic, Anxiolytic and Hypoglycemic Potential in Traditional Medicinal Contexts”. Asian Journal of Research in Botany 7 (2):235-46. https://journalajrib.com/index.php/AJRIB/article/view/223.