In vitro Antioxidant Activities of Aqueous Extracts of Melia azedarach L and Vernonia amygdalina Del.
Manish Kumar *
Department of Botany, SD College, Barnala-148101, Punjab, India.
Sheikh Showkat Ahmad
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab-143005, India.
Amardeep Kaur
Department of Botany, SD College, Barnala-148101, Punjab, India.
Sandeep Kaur
Department of UCRD (University Center for Research and Development), Chandigarh University, Mohali, Punjab -140413, India.
Satwinderjeet Kaur
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab-143005, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: The present study was undertaken to evaluate the antioxidant potential of aqueous extracts of Melia azedarach (AEMA) and Vernonia amygdalina (AEVA) against free radical-induced oxidative damage.
Methodology: The antioxidant activities of the extracts were assessed through three in vitro assays, namely DPPH free radical scavenging assay, ABTS cation radical scavenging assay, and superoxide anion radical scavenging assay.
Results: The findings revealed that both AEMA and AEVA exhibited moderate scavenging activity in the DPPH assay, with IC50 values of 149.83 µg/ml and 198.80 µg/ml, respectively. In the ABTS assay, AEMA and AEVA showed IC50 values 83.38 µg/ml and 135.01 µg/ml respectively. In the case of the superoxide anion radical scavenging assay, AEMA and AEVA demonstrated IC50 values of 130.87 µg/ml and 285.36 µg/ml, respectively.
Conclusion: Overall, the results indicate that Melia azedarach (AEMA) extract was more effective in scavenging free radicals compared to Vernonia amygdalina (AEVA) extract, highlighting its potential for further exploration of in vivo antioxidant efficacy and possible applications in the pharmaceutical and food industries.
Keywords: Melia azedarach, Vernonia amygdalina, antioxidant, DPPH, extracts