Mycelia Colonization Potential of Pleurotus tuber-regium (fr) Singer and Pleurotus ostreatus (Jacq. Ex. Fr) Khum on Different Culture Media and Grain Types
Asian Journal of Research in Botany,
Aim: To determine the effect of different culture media and grain types on the mycelia growth performance of two Pleurotus spp (P. ostreatus and P. tuber-regium).
Study Design: The design this of experiment was a completely randomized design (CRD) with three replications.
Place and Duration of Study: This research was carried out in the Department of Plant and Ecological Studies, University of Calabar, Cross River State, Nigeria, between the period of March –May, 2018.
Methodology: The mycelia colonization potential of these mushrooms were assessed on three culture media viz; potato dextrose agar (PDA), palm inflorescence waste agar (PWA), and spent palm fibre agar (SPFA) and different cereal grain types (viz; Sorghum bisicolor Zea mays, Eleusine coracana, and Triticum aestivum) and a combination of the grains were used as spawn substrates. Parameters assessed include linear mycelium extension, mycelia growth, and mycelia density.
Results: Maximum and minimum mycelia colonization rate of both mushroom species was observed in PDA and PWA, respectively. Results showed that Zea mays and Sorghum bisicolor grains gave the best result in mycelia colonization rate, while Zea mays was poor in supporting mycelia density. The combination of grains at ratio of 1:1 did not contribute positively to the mycelia colonization of P. ostreatus. However, wheat-millet and sorghum-wheat combination at equal proportions greatly enhanced P. tuber-regium mycelia colonization.
Conclusion: This study reveals that PDA was the best medium for culturing both species, while red sorghum was the best grain substrate for P. ostreatus and P. tuber-regium spawn production.
- Culture media
- grain types
- mycelia growth
- Pleurotus ostreatus
- Pleurotus tuber-reguim.
How to Cite
Elhani B, Ansari NA. Effect of substrates of spawn production on the mycelia growth of oyster mushroom species. Journal of Biological Sciences. 2008;8(2):474-477.
Belchew KY, Workie MA. Base substrate sorghum supplied with nitrogen additive-enhanced the proliferation of oyster (Pleurotus ostreatus Jacq. Fr Kummer) mushroom spawn mycelium. International Journal of Science and Research. 2015; 4(3):431-436.
Viziteu G. Substrate-cereal straw and corn cobs. In: Mushroom Growers` Handbook 1, Gush, R. (Ed.). P and F Publishers, USA. 2000;86-90.
Sofi B, Ahmad M, Khan M. Effects of different grains and alternate substrates on oyster mushroom (Pleurotus ostreatus) production. African Journal of Microbiology Research. 2014;8(14):1474-1479.
Mbogoh JM, Anjichi VE, Rotich F, Aloya NK. Substrates effects of grain spawn production on mycelium growth of oyster mushroom. Acta Horticulture. 2011;911: 469-474.
Jayachandran A, Nadesan S, Murugaiyan K. Comparative study of different grains on spawn development of Pleurotus florida. International Journal of Science Invention Today. 2017;6(6):728-735.
Sarita Sharma SS, Somdutt S, Gautam C, Singh R. Optimization of spawn rate for oyster mushroom cultivation in southern Rajasthan. The Pharma Innovation Journal. 2021;10(9):1783-1787.
Mottaghi H. Oyster mushroom and other edible mushrooms. Technology and Producing. Andisheh Farda Publications. 2006;328. (in Farsi).
Chang ST, Hayes WA. (Eds.) The biology and cultivation of edible mushrooms. London, Academic Press, Inc. 1978;819.
Ukoima HN, Ogbonnaya LO, Arikpo GE, Ikper FN Cultural studies of mycelia of Volvareilla volvacea, Pleurotus tuber-regium and Pleurotus sajor-caju on different culture media. Pakistan Journal of Nutrition. 2009;8(7):1052-1054.
Muthu N, Shanmugasundaram K. Effect of five different culture media on mycelial growth of Agrocybe aegerita. International Journal of Pharmaceutical Science and Research. 2015;6(12):5193-5197.
Fasidi IO, Kadiri M. Studies on Pleurotus tuber-regium (Fries) Singer. Use of agricultural waste for the cultivation of Lentinus subnudus in Nigeria. Revista de Biologia Tropical. 1993;41(3):411-415.
Stanley HO, Odu NN, Onwuka JU. The effect of honey on the mycelia growth of Pleurotus sajor cajor (oyster mushroom). International Research Journal of Environ-mental Sciences. 2013;2(12):13-18.
Al Munsur MAZ, Miah A, Rahman MH, Rahman MM, Yahia ASM. Effect of varieties and media on mycelial growth and substrate on spawn production of oyster mushroom. Bangladesh Research Publications Journal. 2012;7(4):361-366.
Maurya AK, John V, Srivastava DK, Sobita Simon S, Pan H. Effect of media and substrates for spawn production of dhingri mushroom (Pleurotus ostreatus). Journal of Natural Resource and Development. 2019;14(2):88-92.
Sardar H, Ali MA, Ayyub MC, Ahmad R. Effects of different culture media, temperature and pH levels on the growth of wild and exotic Pleurotus species. Pakistan Journal of Phytopathology. 2015; 27(2):139-145.
Tudses N. Isolation and mycelia growth of mushrooms on different yam- based culture media. Journal of Applied Biology and Biotechnology. 2016;4(5):33-36.
Kadiri M, Kehinde IA. Production of grain mother and planting spawns of Lentinus subnudus. Nigerian Journal of Botany. 1999;12(1):37-44.
Adebiyi AO, Adebiyi AP, Olaniyi EO. Nutritional composition of Sorghum bicolor starch hydrolysed with amalyse from Rhizopus sp. African Journal of Biotechnology. 2005;4(10):1089-1094.
Sharma S, Yadav RKP, Porkherel CP. Growth and yield of oyster mushroom (Pleurotus ostreatus) on different substrates. Journal of New Biological Reports. 2013;2(1):3-8.
Chandrasekara A, Shahidi F. Bioactivities and antiradical properties of millet grains and hulls. Journal of Agriculture and Food Chemistry. 2011;59(17):9563- 9571.
Shah TR, Prasad K, Kumar P. Maize- A potential source of human nutrition and health. A Review. Cogent Food and Agriculture. 2016;2(1):1166995.
Stanley HO. Effects of substrates of spawn production on mycelial growth of oyster mushroom species. Agriculture and Biology Journal of North America. 2010; 1(5):817-820.
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