In-vitro Antioxidant Screening of Ethanol Extracts of Costus afer and Justicia carnea Leaves
Asian Journal of Research in Botany,
This study evaluated the in-vitro antioxidant activity of ethanol extract of Costus afer and Justicia carnea leaves. Ethanol extract of the plant leaves were obtained using standard procedures. The antioxidant parameters of the plants extract studied were 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, Ferric Reducing Antioxidant Power (FRAP) and Nitric oxide. In each case, the result of test was compared with that of a standard Ascorbic Acid (Vitamin C). The antioxidant study showed that Justicia carnea extract had a significantly higher Nitric oxide radical scavenging ability (04.16 ± 0.68) compared with Costus afer (02.15 ± 0.26) at 50 µg/ml concentration while DPPH scavenging ability of the extract of Justicia carnea showed no significant difference with Costus afer (07.63 ± 0.42) and (06.29 ± 0.53) at 50 µg/ml concentrations respectively. For FRAP test, the result indicated that ethanol extract of Costus afer (01.78 ± 0.22) was significantly lower (p<0.05) than that of Justicia carnea (05.12 ± 0.22) at 800 µg/ml concentration. The findings show that ethanol extracts of Costus afer and Justicia carnea could operate as main antioxidants and free radical scavengers. The findings back up local assertions that they can be used to treat malaria in folklore medicine.
- Costus afer
- Justicia carnea
- medicinal plants
How to Cite
Ameh SJ, Obodozie OO, Inyang SU, Abubakar SM, Garba M. Current phytotherapy; A perspective on the science and regulation of herbal medicine. Journal of Medicinal Plants Research. 2010;4:72-81.
Ige SF, Akhigbe RE, Olaleye SB, Adeyemi JW. Gastroprotective of the methanolic extract of Garcinia kola in rats. International Journal of Medicine and Biomedical Research. 2012;1:172-178
Abdalla AE. The role of antioxidant (Vitamin E) in the control of lead pollution and enhancement of growth within Nile tilapia (Oreochromisniloticus). International Journal of Applied Research Veterinary Medicine. 2009;3: 97-101
Sataro G, Zsolt R. Implications of oxidative damage to proteins and DNA in aging and its intervention by caloric restriction and exercise. Journal of Sport and Health Science. 2013;2(2):75-80
Nwaogu LA, Alisi CS, Ibegbulem CO, Igwe CU. Phytochemical and antimicrobial activity of ethanolic extract of Landophia Oweriensis Leaf. African Journal Biotechnology. 2007;6(7): 890-893.
Paulo A, Duarte A, Gomes ET. In vitro antibacterial screening of Cryptolepiss anguinolenta alkaloids. Journal of Ethnopharmacol. 1994;44:127-130.
Murray A. Dietary reference intake for antioxidant nutrients. Journal of the academy of nutrition and dietetics. 1998; 100:637-640.
Oliver B. Ibadan: Nigerian College of Arts, Sci and Tech, University Press Nigeria; Medicinal Plants in Nigeria. 1960:1–33.
Awouters F, Niemegeers CJ, Lenaerts FM, Janssen PA. Delay of castor oil diarrhoea in rats: A new way to evaluate inhibitors of prostaglandin biosynthesis. Journal of Pharmacy and Pharmacology. 1978;30(1): 41–45.
Chattopadhyay RR, Bandyopadhyay M. Effect of Azadirachta indica leaf extract on serum lipid profile changes in normal and streptozotocin induced diabetic rats. African Journal of Biomedical Research. 2005;8:101–104.
Correa GM, Alcantara A. Chemical constituents and biological activities of species of Justicia: A review. Brazilian Journal of Pharmacognosy. 2012;22(1):220-238.
Badami S, Aneesh R, Sankar S, Sathishkumar MN, Suresh B, Rajan S. Antifertility activity of Derris brevipes variety coriacea. Journal Ethnopharmacology. 2003;84:99–104.
Iwu MM. Traditional Igbo Medicine. Applied Sciences. Institute of Africa Studies Publication, University of Nigeria, Nsukka. 2009;3(4):21-25.
Anaga AO, Njoku CJ, Ekejiuba ES, Esiaka MN, Asuzu IU. Investigations of the methanolic leaf extract of Costusafer. Ker for pharmacological activities in vitro and in vivo. Phytomedicine 2004;11(2-3):242–248.
Hussain W, Badshah L, Ullah M. Quantitative study of medicinal plants used by communities residing in Koh-e-Safaid Range, northern Pakistani-Afghan borders. Journal of Ethnobiology and Ethnomedicine 2018;14:30.
Awuchi CG. Medicinal plants: The medical, food, and nutritional Biochemistry and uses. International Journal of Advanced Academic Research Sciences, Technology and Engineering. 2019;5(11).
Ahmad SU, Shuid AN, Isa NM. Antioxidant and anti-inflammatory activities of Marantodes pumilum (blume) kuntze and their relationship with the phytochemical content. Records of Natural Products. 2018;12(6):518.
Ndukwe GI, Clark PD, Jack IR. In vitro antioxidant and antimicrobial potentials of three extracts of Amaranthus hybridus L. leaf and their phytochemicals. European Chemical Bulletin. 2020;9(7): 164-173.
Rodriguez QA, Costa HS. Analysis of carotenoids in vegetable and plasma sample: A review. Journal of food composition and analysis. 2006;19: 97-111
Jack IR, Clark PD, Ndukwe GI. Evaluation of phytochemical, antimicrobial and antioxidant capacities of Pennisetum purpureum (Schumach) extracts. Chemical Science International Journal. 2020;29(4):1-14.
Anyasor GN, Odunsanya K, Ibeneme A. Hepatoprotective and in vivo antioxidant activity of Costus afer leaf extract against acetaminophen-induced hepatotoxicity in rats. Journal of Investigative Biochemistry. 2013;2:53–61.
Ukpabi CF, Agbafor KN, Ndukwe OK, Agwu A, Nwachukwu SN. Phytochemical Composition of Costus Afer Extract and Its Alleviation 122 of Carbon Tetrachloride – induced Hepatic Oxidative Stress and Toxicity. International Journal of Modern Botany. 2012;2:120-126.
Hemali P, Anjali MT, Sumitra C. Antimicrobial activity of some medicinal plant extracts and its synergistic interaction with some antibiotics. Journal of Pharmacy Research. 2016;10 (5): 211-220
Baskar R, Rajeswari V, Kumar TS. In-vitro antioxidant studies in leaves of Annona species Indian Journal of Experimental Biology. 2007;45(5) 480-485.
Houcine B, Asma B, Faiza M, Abdelillah A. Free radical scavenging activity, kinetic behavior and phytochemical constituents of Aristolochia clematiti L. roots. Arabian Journal of Chemistry. 2017;10(1):S1402-S1408.
Okawa M, Kinjo J, Nohara T, and Ono, M. DPPH (1, 1-diphenyl-2- picrylhydrazyl) radical scavenging activity of flavonoids obtained from some medicinal plants. Biological and Pharmaceutical Bulletin: 2001;24:1202-1205.
Zhiyong C, Yuanzong L. Reducing power: the measure of antioxidant activities of reductant compound. Redox Report. 2004;9:4:213-217.
Satish BN, Dilipkumar P. Free radicals, natural antioxidants, and their reaction mechanisms. Royal Society of Chemistry. 2015;5:27986-28006.
Benzie IF, Strain JJ . The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Analytical Biochemistry. 1996;239: 70–76.
Kalu O, Igwe CO, Ujowundu SC, Chukwudoruo, Obasi UK. Assessment of hematological and serum electrolyte of albino rat administered with graded concentration of ethanol extract of ficus capensis. Asian Journal of research in Botany. 2020;4(3):28-36
Mensor LL, Fabio SM, Gilda GL, Alexandre SR, Teredza CD, Cintia SC, Suzana GL. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research. 2001;15:127-130.
Omokhua GE. Medicinal and socio-cultural importance of Costus Afer (Ker Grawl) in Nigeria. African Research Review. 2011;5:282–7.
Katia A, Sara B, Simone C, Giuseppe R, Maria RC. Nitric oxide is the primary mediator of cytotoxicity induce GSH depletion in neuronal cells. Journal of Cell Science. 2011;124:1043-1054
Abstract View: 52 times
PDF Download: 15 times