Determining the Best Solvent for Extracting Soluble Sugars; Proteins and Amino Acid Content from Moringa oleifera Lam Leaf Powder Grown in Libya
Issue: 2021 - Volume 4 [Issue 2]
Khadija M. Misratia *
Department of Biology, Faculty of Education Misurata University, Libya.
Rabha M. Mansur *
Department of Botany, Faculty science, Omar Al-Mukhtar University, Al Bayda, Libya.
*Author to whom correspondence should be addressed.
Moringa oleifera Lam has been used in traditional medicines since long time worldwide. Sugars; proteins and amino acid assumed to be responsible for many of the biological activities in plant cell. The effects of different extraction solvents, on biochemical content of Moringa oleifera Lam leaf powder have been investigated. The highest sugar content was by extracting it with an Methyl alcohol, where it reached 1.89 (mg g-1 DW) followed by Ethanol extract (1.66 mg g-1 DW) However, distilled water extract recorded the lowest content of soluble sugars compared to Methyl and Ethanol alcohol extraction, where it reached (1.30 mg g-1 DW). The results indicated that the high soluble proteins content recorded with Methyl alcohol and distilled water (0.255 and 0.168 mg g-1 DW). On the other hand, the low soluble proteins content with Ethanol alcohol compared to other extracts. high amino acids content with Methyl alcohol (0.55 mg g-1 DW) and distilled water record 0.47 mg g-1 DW, while the low of amino acids content (0.27 mg g-1 DW) it was with the Ethanol alcohol extract.
Keywords: Amino acids, ethanol, organic solvents, methyl alcohol, moringa oleifera and soluble sugars