Asian Journal of Research in Botany

In vivo and In vitro Initiatives for the Examination of Pharmacological Properties of Brassaiopsis hainla Leaves

Shafiul Azam Zishan

Department of Pharmacy, International Islamic University Chittagong, Kumira-4318, Chittagong, Bangladesh.

Md. Maien Uddin

Department of Pharmacy, International Islamic University Chittagong, Kumira-4318, Chittagong, Bangladesh.

Mahathir Mohammad

Department of Chemistry, Chittagong University of Engineering and Technology, Chittagong-4349, Bangladesh.

Mohammad Eshmam Uddin

Department of Pharmacy, International Islamic University Chittagong, Kumira-4318, Chittagong, Bangladesh.

S. M. Asadul Karim Azad

Department of Pharmacy, International Islamic University Chittagong, Kumira-4318, Chittagong, Bangladesh.

Jannatul Naima

Department of Pharmacy, University of Chittagong, Chittagong- 4331, Bangladesh.

Sadab Sipar Ibban

Department of Pharmacy, International Islamic University Chittagong, Kumira-4318, Chittagong, Bangladesh and Department of Pharmacy, University of Chittagong, Chittagong- 4331, Bangladesh.

*Author to whom correspondence should be addressed.

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Abstract

Aims: This study is intended to evaluate the methanol extract of Brassaiopsis hainla leaves (MEBH) in the parameter of helminths, thrombolytic, cytotoxic, diarrhea & pyrexia by using In vivo and in vitro tactics.

Methodology: In vivo tests, including those for anti-diarrheal and antipyretic effects, were conducted using mice that were orally dosed with MEBH, while in vitro tests were conducted using aquarium worms, human blood, and brine shrimp for a specific purpose, such as anti-helminthic analysis, clot lysis analysis, and cytotoxicity.

Results: BRASSAIOPSIS HAINLA have showed a potential effect in certain diseases, the anthelmintic potential of MEBH was demonstrated in a dose-dependent approach on aquatic worms (Tubifex Tubifex) at different concentrations. Still, the effective one is MEBH 100 (µg/ml) = 29.749 ± 1.444 times of paralysis, 15.256 ± 6.118. The MEBH demonstrated a high level of thrombolytic activity at 48.358% of clot lysis. Compared to negative control but lower than standard drug. Following 24 hours, the LC₅₀ was assessed in a brine shrimp lethality test. The LC₅₀ value of the extract was 328.02 µg/mL, which is relatively toxic to brine shrimp, predominantly when associated with the LC₅₀ value of 3.8 µg/mL for typical vincristine sulfate. In the research examining its efficacy towards diarrhea, Gastrointestinal motility, and peristalsis indexes among 200 and 400 mg/kg were identified to be significantly (P < 0.005) hindered by MEBH. The highest diminution in fever elicited by MEBH at 400 mg/kg demonstrates its promising antipyretic potential.

Conclusion: Our study confirmed that MEBH possesses anthelmintic, antipyretic, cytotoxic, thrombolytic, and anti-diarrheal effects, which makes it an intriguing source for innovative treatments. This could be an alternative remedy for common diseases that have to take synthetic medication.

Keywords: Anthelmintic, Anti-diarrheal, Brassaiopsis hainla (MEBH), brine shrimp lethality bioassay, methanolic extract, thrombolytic

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